Spectroscopy Ii- Hplc & Raman

UNIVERSITI TUNKU ABDUL RAHMAN Faculty Course category/ Semester Session : : : : Faculty of acquaintance Bac. of Science (Hons) Chemistry twelvemonth 3 Semester 1 201105 or May 2011 Unit write in code Unit Title ref Assignment : : : : UDEC3254 Spectroscopy and Chromatography II Dr. Lim Chan Kiang 2 Answer t bring disclose ensemble questions 1. A categorization of steroid hormone hormones consisting of cortisone, cortexone and corticosterone was separated using a HPLC silica editorial eluted with a binary mixture of dimethyl ketone and hexane (20:80). (i) What is the differentiate of elution time for the in a higher place escalates from the HPLC column? (3 marks) (ii) What is the chromatographic chemical utensil involved? get out how the mechanism effects detachment to the threesome compounds. (8 marks) (iii) With explanation, describe what would pass to the peaks in the chromatogram when the packing silica with a larger revolve about size and smaller come to the fore stadium is used in the above separation. (6 marks) 2. A mixture of Br-, Cl- and PO43- ions was subjected to an non-ionic detergent column jam-packed with polym-N(CH3)3+OH-, eluted with 9mM sodium carbonate. (i) Arrange the above analyte ions in order of change magnitude retention time in the anionic detergent column.

(3 marks) (ii) get word how the analyte ions were separated in the anionic column harmonise to order of retention time answered in part (2)(i). (8 marks) 3. An stranger compound is irradiated with an exhilaration photon. An anti-Stokes pull out is observed at 439.3 nm corresponding to shift of 2270 cm-1. i) What is the excitation photon used in the guesswork above? (2 marks) ii) Find the wavenumber and wavelength of the Stokes railway and anti-Stokes contention with shifts of 3270 cm-1 and 1170 cm-1, respectively. (6 marks) iii) A Stokes line supposed to be launch in the region 490 nm to 500 nm in the Raman spectrum was swamped by fluorescent band. unwrap how to solve the problem so that the Raman shift for the Stokes line direct out be determined. (4 marks) If you fate to get a all-inclusive essay, order it on our website: Ordercustompaper.com

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